Journal: Animals : an Open Access Journal from MDPI

Article Title: Establishment and Characterisation of Two Canine Prostate Cancer Cell Lines with Stem Cell Marker Expression

doi: 10.3390/ani16050732

Figure Lengend Snippet: Comparison of the expression of CK8/18, CK14, p63 and UPIII in the Kodiak original tumour and mouse xenograft tumour as evaluated using immunohistochemistry (IHC) and the cell line grown in MesenCult TM MSC Medium as evaluated using immunofluorescence (IF). Bar for tumour and xenograft IHC = 10 µm. Bar for cell line IF = 40 µm. IHC: DAB chromagen (positive cells = brown); IF: DAPI (blue nuclear staining), fluorescein (green positive cells).

Article Snippet: CK8/18 , Novocastra (Leica Biosystems, Nussloch, Germany) , 5D3 , MM , 1:600 , Proteinase K (5 min incubation) , ImmPRESS ® HRP Universal Polymer Kit (Peroxidase) or VECTASTAIN ® Elite ® ABC-HRP Kit (Peroxidase) , Canine mammary tumour and/or normal canine skin.

Techniques: Comparison, Expressing, Immunohistochemistry, Immunofluorescence, Staining

Journal: Animals : an Open Access Journal from MDPI

Article Title: Establishment and Characterisation of Two Canine Prostate Cancer Cell Lines with Stem Cell Marker Expression

doi: 10.3390/ani16050732

Figure Lengend Snippet: Comparison of the expression of CK8/18 and CK14 in the Bobby original tumour as evaluated using immunohistochemistry (IHC) and cell line grown in MesenCult TM MSC Medium (MSC) and PrEGM TM Prostate Epithelial Cell Growth Medium (PrEGM) as evaluated using immunofluorescence (IF). Bar for tumour IHC = 10 µm. Bar for cell line IF = 40 µm. IHC: DAB chromagen (positive cells = brown); IF: DAPI (blue nuclear staining), fluorescein (green positive cells).

Article Snippet: CK8/18 , Novocastra (Leica Biosystems, Nussloch, Germany) , 5D3 , MM , 1:600 , Proteinase K (5 min incubation) , ImmPRESS ® HRP Universal Polymer Kit (Peroxidase) or VECTASTAIN ® Elite ® ABC-HRP Kit (Peroxidase) , Canine mammary tumour and/or normal canine skin.

Techniques: Comparison, Expressing, Immunohistochemistry, Immunofluorescence, Staining

Journal: iScience

Article Title: DACIT device for axon cancer cell interaction testing in 2D and 3D

doi: 10.1016/j.isci.2025.114557

Figure Lengend Snippet: DACIT enables media compartmentalization or diffusion and supports cellular compartmentalization (A) Fluidic compartmentalization (left: 200 μL of media loaded in the neuronal compartment and 150 μL in the axonal compartment) or free diffusion (right, 200 μL loaded in each compartment) were monitored 0–48 h, via 3 kDa fluorescent dextran signal. Dextran diffuses from the axonal compartment in a retrograde fashion, moving across the microgrooves and into the neuronal compartment. Pseudocolor 0–700 μm maximum projections (top, legend on the right) show dextran distribution in microchannels and microgrooves at 24 h. Note that the signal from the microgrooves (3 μm in height), compared to the signal from the macrochannels (700 μm in height), is expected to be approximately 700/3 = 233-fold higher, appearing similar to the background level. Bottom panels show dextran distribution over the entire course of 0–48 h (1 h interval). Readings from the axonal compartment are shown as red dotted lines and from the neuronal compartment as black dotted lines. Scale bars, 200 μm. (B) PC12-GFP or PGP9.5-labeled primary DRG cultures after 3 days in culture. Fluorescence was pseudocolored black for clarity. Left: close-up of axons extending across the microgrooves. Scale bars, 100 μm. Right: overview showing axons populating the axonal compartment. Scale bars, 200 μm. (C) Quantification of axonal length in PC12 and primary DRG cultures from five fields of view. (D) Inverted immunofluorescence images of monocultures of BT-549 breast cancer cells (F-actin, top) or sensory neurons (PGP9.5, bottom) fixed on day 3 post-loading in DACIT. Scale bars, 200 μm. (E) Immunofluorescence showing single channels in grayscale (left) and fluorescent overlay image (right). Neurons are labeled with PGP9.5 (white), Schwann cells (SC) with Sox-10 (yellow), BT-549 cancer cells (CC) with CK8/18 (magenta), and nuclei with DAPI (cyan). Neuronal soma is pointed with yellow arrows (left). Scale bars, 200 μm.

Article Snippet: Diluted 1:100); CK8/18 (PROGEN, Cat#GP11.

Techniques: Diffusion-based Assay, Labeling, Fluorescence, Immunofluorescence